試劑耗材
Viromer來自德國,為創(chuàng)新型轉(zhuǎn)染試劑而生,且只做轉(zhuǎn)染試劑,只賣轉(zhuǎn)染試劑,因為專業(yè),所以值得信賴!
如果您需要轉(zhuǎn)染的細胞非常難轉(zhuǎn),多次轉(zhuǎn)染不成功的話,建議嘗試Viromers®轉(zhuǎn)染試劑,它是基于病毒融合機制的聚合物,該機制以流感病毒血凝素的融合肽為藍本;當暴露于低pH時,融合肽失電荷,變?yōu)槭杷,進入核膜。推薦用于原代細胞,懸浮細胞,分化中細胞或干細胞等難轉(zhuǎn)細胞。Viromers® Green/Blue均適用于siRNA和miRNA轉(zhuǎn)染,分別適用于不同的細胞系(貼壁細胞或懸浮細胞),均表現(xiàn)出高轉(zhuǎn)染效率。
Viromers®優(yōu)點:- 避免包涵體吞噬:最大化轉(zhuǎn)染效率并降低背景;
- 零電荷:無需去除血清和抗生素,對細胞溫和;
- 無脂質(zhì):適用于脂肪代謝活躍的細胞,如:脂肪細胞或肝細胞。
- 穩(wěn)定:實驗結(jié)果具有重復性;
- 反向轉(zhuǎn)染:適用于高通量篩選。
使用Viromer®BLUE /GREEN , Lipofectamine轉(zhuǎn)染siRNA 到原代小鼠成纖維細胞中,檢測目標基因和GAPDH mRNA的表達
使用Viromer®BLUE /GREEN , Lipofectamine轉(zhuǎn)染siRNA 到cancer stem cells / mammospheres中,檢測目標基因和GAPDH mRNA的表達
反向轉(zhuǎn)染表現(xiàn)優(yōu)越,適用于高通量篩選
Viromer®BLUE轉(zhuǎn)染原代巨噬細胞,在384孔板中,使用Viromer®BLUE轉(zhuǎn)染siRNA targeting EEA1到原代巨噬細胞(從buffy coat PBMC 現(xiàn)分離)中,檢測endosomes 水平(siRNA targeting EEA1會導致endosomes 水平降低)
訂購詳情:
表. Viromer® Blue and Green cell preferences貨號 品名 規(guī)格 價格 備注 VB-01LB-00 Viromer® BLUE 80 T in 24 well 1000 已驗證細胞系
參照表6VB-01LB-01 0.3 ml(500T) 5580 VB-01LB-03 3 x 0.3 ml(1500T) 14160 VG-01LB-00 Viromer® GREEN 80 T in 24 well 1000 VG-01LB-01 0.3 ml(500T) 5580 VG-01LB-03 3 x 0.3 ml(1500T) 14160 Category Cell type Viromer® Cell lines Standards: HeLa, Neuro2A, CHO, A549, HepG2, HEK-293 BLUE &GREEN MCF-7 breast adenocarcinoma BLUE RAW264.7 & NIH3T3 BLUE U87, NCH82 & NCH149 glioblastoma BLUE &GREEN MDA-MB468 &-MB231 breast adenocarcinoma BLUE &GREEN CT26, HT-29, HCT116 colon carcinoma BLUE &GREEN P4E6 prostate carcinoma BLUE Hs746T gastric carcinoma BLUE &GREEN MPC mouse pheochromocytoma cells BLUE &GREEN SH-SY5Y neuroblastoma GREEN MRC-5 lung fibroblasts GREEN TEU-2 immortilizedurothelial cells GREEN C2C12 myoblasts BLUE U2OS osteosarcoma BLUE Suspension cells THP-1 monocytes BLUE &GREEN CHO-K1 suspension GREEN Kasumi-4 & MV4-11 myelomonocytic leukemia cells BLUE Primary & Stem cells Pre-adipocytes (3T3-L1, C3H10T1/2) & adipocytes BLUE Schwann cells from mouse brain BLUE Primary mouse hepatocytes BLUE Primary human macrophages & PBMCs BLUE Primary fibroblasts BLUE &GREEN Primary human skeletal myoblasts BLUE Primary human bladder smooth muscles BLUE HUVECs BLUE Cancer stem cells / 3D-mammospheres BLUE Dental follicle stem cells GREEN Special applications 3D-mammosphere / Co-culture transfection BLUE Disaggregated primary human xenograft tumor cells BLUE